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KMID : 1098420050130010057
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 1 p.57 ~ p.62
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Transformation of Korean Ginseng (Panax ginseng C.A. Meyer) with Salt Toleranc SAL1 Gene
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In Jun-Gyo
Yang Deok-Chun
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Abstract
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Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 geue (3’’(2’’), 5’’-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 mg/l cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 mg/l kanamycin and 250 mg/l cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 mg/l kanamycin and 10 mg/l gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.
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KEYWORD
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Agrobacterium, Panax ginseng, SAL1, salt tolerance, somatic embryo
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